human melanoma cell line m10 Search Results


human melanoma cell line m10  (ATCC)
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ATCC human melanoma cell line m10
Figure 6. Analyses of kindlin-3 expression in <t>M10</t> human melanoma cell line. (A) qRT-PCR analyses of kindlin-1, ¡2 and ¡3 mRNA expression levels in M10 cells and the human chronic myeloid leukemia cell line K562. Values are mean § SD of technical triplicates. A representative experiment from 3 independent experiments is shown. (B) qRT-PCR analyses of kindlin-3 expression in M10, K562 and the human fibroblast cell line 293T. In (A) and (B), GAPDH and actin were used as internal controls. (C) Immunoblot analyses of kindlin-3 protein expression in M10 cells, K562, 293T and the human breast cancer cell line MDA-MB-231 using the anti-kindlin-3 mAb clone 9. Actin serves as loading control. (D) Immunoblot analyses using the anti-kindlin-3 mAb 3D6.
Human Melanoma Cell Line M10, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Analyses of kindlin-3 expression in M10 human melanoma cell line. (A) qRT-PCR analyses of kindlin-1, ¡2 and ¡3 mRNA expression levels in M10 cells and the human chronic myeloid leukemia cell line K562. Values are mean § SD of technical triplicates. A representative experiment from 3 independent experiments is shown. (B) qRT-PCR analyses of kindlin-3 expression in M10, K562 and the human fibroblast cell line 293T. In (A) and (B), GAPDH and actin were used as internal controls. (C) Immunoblot analyses of kindlin-3 protein expression in M10 cells, K562, 293T and the human breast cancer cell line MDA-MB-231 using the anti-kindlin-3 mAb clone 9. Actin serves as loading control. (D) Immunoblot analyses using the anti-kindlin-3 mAb 3D6.

Journal: Cell adhesion & migration

Article Title: Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis.

doi: 10.1080/19336918.2016.1243645

Figure Lengend Snippet: Figure 6. Analyses of kindlin-3 expression in M10 human melanoma cell line. (A) qRT-PCR analyses of kindlin-1, ¡2 and ¡3 mRNA expression levels in M10 cells and the human chronic myeloid leukemia cell line K562. Values are mean § SD of technical triplicates. A representative experiment from 3 independent experiments is shown. (B) qRT-PCR analyses of kindlin-3 expression in M10, K562 and the human fibroblast cell line 293T. In (A) and (B), GAPDH and actin were used as internal controls. (C) Immunoblot analyses of kindlin-3 protein expression in M10 cells, K562, 293T and the human breast cancer cell line MDA-MB-231 using the anti-kindlin-3 mAb clone 9. Actin serves as loading control. (D) Immunoblot analyses using the anti-kindlin-3 mAb 3D6.

Article Snippet: Human melanoma cell line M10 was purchased from American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 fullmedium containing supplements aforementioned.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Figure 7. Analyses of the migratory properties of M10 cells transfected with GFP-kindlin-3. (A) Flow cytometry analyses and sorting of cells transiently transfected with GFP or GFP-kindlin-3 expression plasmid. (B) Sorted cells were subjected to 2D-random migration assays on fibronectin-coated (2 mg/cm2) coverslip-bottom culture dishes. Twenty cells were tracked and migratory profiles were plotted. Representative data from at least 2 independent experiments are shown. X and Y axes are relabeled with larger fonts for clarity.

Journal: Cell adhesion & migration

Article Title: Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis.

doi: 10.1080/19336918.2016.1243645

Figure Lengend Snippet: Figure 7. Analyses of the migratory properties of M10 cells transfected with GFP-kindlin-3. (A) Flow cytometry analyses and sorting of cells transiently transfected with GFP or GFP-kindlin-3 expression plasmid. (B) Sorted cells were subjected to 2D-random migration assays on fibronectin-coated (2 mg/cm2) coverslip-bottom culture dishes. Twenty cells were tracked and migratory profiles were plotted. Representative data from at least 2 independent experiments are shown. X and Y axes are relabeled with larger fonts for clarity.

Article Snippet: Human melanoma cell line M10 was purchased from American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 fullmedium containing supplements aforementioned.

Techniques: Transfection, Flow Cytometry, Expressing, Plasmid Preparation, Migration